Stranded driftwood feedstocks may represent, after pretreatment with steam explosion and enzymatic hydrolysis, a cheap C-source for producing biochemicals and biofuels using oleaginous yeasts.The hydrolysis was optimized using a response chicago bears garden gnome surface methodology (RSM).The solid loading (SL) and the dosage of enzyme cocktail (ED) were variated following a central composite design (CCD) aimed at optimizing the conversion of carbohydrates into lipids (YL) by the yeast Solicoccozyma terricola DBVPG 5870.A second-order polynomial equation was computed for describing the effect of ED and SL on YL.The best combination (ED = charger rt procharger 3.
10%; SL = 22.07%) for releasing the optimal concentration of carbohydrates which gave the highest predicted YL (27.32%) was then validated by a new hydrolysis.The resulting value of YL (25.26%) was close to the theoretical maximum value.
Interestingly, fatty acid profile achieved under the optimized conditions was similar to that reported for palm oil.Keywords: Stranded driftwood feedstocks, Solicoccozyma terricola, Enzymatic hydrolysis, Yeast biochemicals and biofuels, Response surface methodology.